Inverted duplicate DNA sequences increase translocation rates through sequencing nanopores resulting in reduced base calling accuracy.
作者： Pieter Spealman , Jaden Burrell , David Gresham
DOI: 10.1093/NAR/GKAA206
关键词: Inverted Repeat Sequences 、 Copy-number variation 、 Biology 、 Sequence analysis 、 Computational biology 、 DNA sequencing 、 Base calling 、 Nanopore 、 Nanopore sequencing 、 DNA
摘要: Inverted duplicated DNA sequences are a common feature of structural variants (SVs) and copy number (CNVs). Analysis CNVs containing inverted using nanopore sequencing identified recurrent aberrant behavior characterized by low confidence, incorrect missed base calls. duplicate in both yeast human samples were observed to have systematic elevation the electrical current detected at nanopore, increased translocation rates decreased sampling rates. The coincidence with dramatically reduced accuracy an rate suggests that secondary structures may interfere dynamics transit through nanopore.
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