Ex vivo expansion of human umbilical cord blood hematopoietic progenitor cells in a novel three-dimensional culture system
作者： Fuyin Xiong , Zhaolie Chen , Hong Liu , Zhaoping Xu , Xingmao Liu
关键词: Progenitor cell 、 Umbilical cord 、 Stromal cell 、 CD34 、 Molecular biology 、 Haematopoiesis 、 Cell culture 、 Immunology 、 Bone marrow 、 Biology 、 Peripheral blood mononuclear cell
摘要: A novel three-dimensional culture system for the ex vivo expansion of human umbilical cord blood (CB) hematopietic progenitor cells (HPCs) was developed by growing CB mononuclear on highly porous CultiSpher G microspheres coated with bone marrow stromal in stirred flasks presence supplemented cytokines. After 12 days, number total viable cells, colony-forming units (CFU-C) and CD34+ present cultures reflected average increases 7.7, 23.3 9.6-fold, respectively, marked hematopoietic islands were formed surface G.
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