Methylene blue photodynamic therapy in malignant melanoma decreases expression of proliferating cell nuclear antigen and heparanases.
作者： M. Wagner , E. R. Suarez , T. R. Theodoro , C. D. A. S. Machado Filho , M. F. M. Gama
DOI: 10.1111/J.1365-2230.2011.04291.X
关键词: Proliferating cell nuclear antigen 、 Photodynamic therapy 、 Downregulation and upregulation 、 Experimental pathology 、 Melanoma 、 Biology 、 Heparanase 、 Molecular biology 、 Reactive oxygen species 、 Immunohistochemistry
摘要: Summary Background. Malignant melanoma (MM) is a very aggressive tumour. Although surgical excision of MM in the early stages has good prognosis, it often fails to completely inhibit tumour progression. Methylene blue photodynamic therapy (MB-PDT) technique that induces tissue damage by reactive oxygen species (ROS). Aim. To investigate efficacy and potential use MB-PDT restraining aggressiveness analysing levels proliferating cell nuclear antigen (PCNA) heparanase (HPSE, molecular marker invasion) mouse model. Methods. Expression PCNA two HPSE isoforms were analysed using immunohistochemistry (IHC) after mice. Tumour volume weight also measured. Results. Two treatments with promoted decrease 99% 75% compared untreated mice (P < 0.05). Using IHC, expression for 95% both (P < 0.05) was found. Conclusion. cheap efficient method decreasing thus disease This reduction mediated downregulation heparanases.
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