A convenient and versatile method for the purification of CoA thiol esters
作者： Maynard E. Pullman
DOI: 10.1016/0003-2697(73)90262-5
关键词: Acetaldehyde 、 Adenine nucleotide 、 Thiol 、 Chromatography 、 Alcohol oxidoreductase 、 Chemistry 、 Analytical procedures 、 Resolution (mass spectrometry) 、 Spectrophotometry 、 Structure–activity relationship 、 Biophysics 、 Cell biology 、 Biochemistry 、 Molecular biology
摘要: Abstract A simple and versatile chromatographic procedure has been described for the purification of fatty acyl-CoA thiol esters, ranging in chain length from C 2 to 18 . The method yields products that are over 90% pure, as judged by several criteria, 80% or greater. It may also be applicable analytical procedures where resolution isolation acylated CoA, CoASH, CoA-S-S-CoA adenine nucleotide a large number reaction mixtures is required.
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