Regulation of enzymes by fatty acyl coenzyme A. Site-specific binding of fatty acyl coenzyme A by citrate synthase--a spin-labeling study.
作者： Gary L. Powell , Anthony V. Caggiano
DOI: 10.1016/S0021-9258(17)30144-8
关键词:
摘要: 4.1.3.7) at about the same concentration as did palmitoyl-CoA. Competitive binding studies show acetyl-CoA and ATP to displace 6- 16-doxylstearoyl-CoA from citrate synthase but little while oxalacetate NADPH compete very effectively. These may suggest by fatty acyl-CoA sites apart site. The differential displacement argues against of a general nucleotide spectrum 6-doxylstearoyl-CoA in presence high concentrations was for an immobilized spin label; under conditions mobile. spectral differences were interpreted terms specific site which portion acyl chain proximal thioester methyl end allowed greater motional freedom. Phospholipid vesicles included label not that 6doxylstearoyl-CoA excess support above interpretation. A model on with provision interaction biological membrane is presented. Site-specific provides strong physiological role regulation analogy other related enzymes lipid metabolism.
sci-hub.st 参考文章(44)
Jacque Monod, Jeffries Wyman, Jean-Pierre Changeux, On the Nature of Allosteric Transitions: A Plausible Model Journal of Molecular Biology. ,vol. 12, pp. 88- 118 ,(1965) , 10.1016/S0022-2836(65)80285-6
Philippe F. Devaux, Alain Bienvenue, Guy Lauquin, Alain D. Brisson, Paulette M. Vignais, Pierre V. Vignais, Interaction between spin-labeled acyl-coenzyme A and the mitochondrial adenosine diphosphate carrier Biochemistry. ,vol. 14, pp. 1272- 1280 ,(1975) , 10.1021/BI00677A028
Carl-Johan JOHANSSON, Gosta PETTERSSON, Kinetics of the Inhibition of Citrate Synthase from Pig Heart by Substrate Analogues European Journal of Biochemistry. ,vol. 46, pp. 5- 11 ,(1974) , 10.1111/J.1432-1033.1974.TB03591.X
W. L. Hubbell, H. M. McConnell, Spin-label studies of the excitable membranes of nerve and muscle. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 61, pp. 12- 16 ,(1968) , 10.1073/PNAS.61.1.12
D. E. Koshland, G. Némethy, D. Filmer, Comparison of Experimental Binding Data and Theoretical Models in Proteins Containing Subunits* Biochemistry. ,vol. 5, pp. 365- 385 ,(1966) , 10.1021/BI00865A047
George L. Ellman, Tissue sulfhydryl groups Archives of Biochemistry and Biophysics. ,vol. 82, pp. 70- 77 ,(1959) , 10.1016/0003-9861(59)90090-6
O. Wieland, L. Weiss, I. Eger-Neufeldt, A. Teinzer, B. Westermann, Coenzym A-Thioester höherer Fettsäuren als mögliche Vermittler enzymatischer Regulationen im Tierkörper Journal of Molecular Medicine. ,vol. 43, pp. 645- 654 ,(1965) , 10.1007/BF01728702
Maynard E. Pullman, A convenient and versatile method for the purification of CoA thiol esters Analytical Biochemistry. ,vol. 54, pp. 188- 198 ,(1973) , 10.1016/0003-2697(73)90262-5
M. Sumper, H. Träuble, Membranes as acceptors for palmitoyl CoA in fatty acid biosynthesis FEBS Letters. ,vol. 30, pp. 29- 34 ,(1973) , 10.1016/0014-5793(73)80612-X
Koji Nishikori, Nobuko Iritani, Shosaku Numa, Levels of acetyl coenzyme A carboxylase and its effectors in rat liver after short-term fat-free refeeding. FEBS Letters. ,vol. 32, pp. 19- 21 ,(1973) , 10.1016/0014-5793(73)80725-2