γ-TuRC Heterogeneity Revealed by Analysis of Mozart1.

摘要: Microtubules are essential for various cell processes [1] and nucleated by multi-protein γ-tubulin ring complexes (γ-TuRCs) at microtubule organizing centers (MTOCs), including centrosomes [2-6]. Recruitment of γ-TuRCs to different MTOCs times influences array formation, but how this is regulated remains an open question. It also unclear whether all within the same organism have composition any potential heterogeneity might influence γ-TuRC recruitment. MOZART1 (Mzt1) was recently identified as a component [7, 8] conserved in nearly eukaryotes [6, 9]. Mzt1 has so far been studied cultured human cells, yeast, plants; its absence leads failures recruitment division, resulting death [7, 9-15]. small (∼8.5 kDa), binds directly core components [9, 10, 14, 15], appears mediate interaction between proteins that tether 15]. Here, we use Drosophila investigate function multicellular animal first time. Surprisingly, find expressed only testes present recruited basal bodies, not mitochondria, developing sperm cells. mzt1 mutants viable defects body positioning centriole adjuncts; sperm formation affected display rapid age-dependent decline motility male fertility. Our results reveal tissue-specific MTOC-specific exist highlight complexity animal.