The Glycosylphosphatidylinositol-Anchored Form and the Transmembrane Form of CD58 Associate with Protein Kinases

摘要: The significance of the glycosylphosphatidylinositol (GPI) anchor is unknown. Since GPI-anchored proteins mediate signaling, it has been suggested that GPI structure serves as a signal-transducing element. However, division signaling functions between transmembrane and unclear. Studies distinct membrane-anchored forms same protein may resolve this issue. adhesion molecule CD58 expressed on cell surface in both form hence provides useful model. We studied human B lymphoblastoid line JY. In addition to mediating adhesion, involved signal transduction. Incubation JY cells with immobilized anti-CD58 Abs results extensive tyrosine phosphorylation secretion TNF-α. demonstrate associated kinase(s) several kinase substrates. further isoforms are involved. variant cells, which express only form, well activity. This association pattern common wild-type cells. Thus, these findings suggest capacity interact kinases not always dependent itself.