T cell receptor tyrosine phosphorylation. Variable coupling for different activating ligands.

摘要: We have previously reported (Samelson, L.E., Patel, M.D., Weissman, A.M., Harford, J.B., and Klausner, R.D. (1986) Cell 46, 1083-1090) that T cell activation by antigen is associated with of two biochemical pathways. In this scheme protein kinases are activated stimulation the receptor (TCR). These phosphorylate different chains TCR complex. Protein kinase C responsible for phosphorylation gamma, and, to a lesser extent, epsilon on serine residues while an unidentified tyrosine leads p21 subunit residues. addition specific antigens, cells can be functionally in vitro antibodies bind either or Thy-1 molecule, entity independent receptor. used directed against these molecules show they result same dual observed stimulation. we compared three ligands, antigen, chain Thy-1, terms how couple Activation phosphatidylinositol breakdown all stimuli sensitive cAMP inhibition. contrast, only antigen-stimulated antibody reagents activate manner entirely insensitive