Epigenetic transcriptional repression of cellular genes by a viral SET protein.
作者： Shiraz Mujtaba , Karishma L. Manzur , James R. Gurnon , Ming Kang , James L. Van Etten
DOI: 10.1038/NCB1772
关键词: Chromatin 、 Histone 、 Genetics 、 Gene 、 Gene silencing 、 Transcription (biology) 、 Cell biology 、 Regulation of gene expression 、 Biology 、 Epigenetics 、 Histone H3
摘要: Viruses recruit host proteins to secure viral genome maintenance and replication. However, whether they modify histones directly interfere with chromatin-based transcription is unknown. Here we report that Paramecium bursaria chlorella virus 1 (PBCV-1) encodes a functional SET domain histone Lys methyltransferase (HKMTase) termed vSET, which linked rapid inhibition of after infection. We show vSET packaged in the PBCV-1 virion, it contains nuclear localization signal probably represses by methylating H3 at 27 (H3K27), modification known trigger gene silencing eukaryotes. also induces cell accumulation G2/M phase recruiting Polycomb repressive complex CBX8 methylated H3K27 site heterologous system, vSET-like have methylation activity are conserved viruses. Our findings suggest mechanism repress direct chromatin vSET.
nature.com
core.ac.uk
elsevier.com
sci-hub.se 参考文章(35)
Bradley E. Bernstein, Tarjei S. Mikkelsen, Xiaohui Xie, Michael Kamal, Dana J. Huebert, James Cuff, Ben Fry, Alex Meissner, Marius Wernig, Kathrin Plath, Rudolf Jaenisch, Alexandre Wagschal, Robert Feil, Stuart L. Schreiber, Eric S. Lander, A Bivalent Chromatin Structure Marks Key Developmental Genes in Embryonic Stem Cells Cell. ,vol. 125, pp. 315- 326 ,(2006) , 10.1016/J.CELL.2006.02.041
Tomek Swigut, Joanna Wysocka, H3K27 Demethylases, at Long Last Cell. ,vol. 131, pp. 29- 32 ,(2007) , 10.1016/J.CELL.2007.09.026
Anke Sparmann, Maarten van Lohuizen, Polycomb silencers control cell fate, development and cancer Nature Reviews Cancer. ,vol. 6, pp. 846- 856 ,(2006) , 10.1038/NRC1991
Anne M. Schuster, Dwight E. Burbank, Barbara Meister, Merri P. Skrdla, Russel H. Meints, Stanley Hattman, David Swinton, James L. Van Etten, Characterization of viruses infecting a eukaryotic Chlorella-like green alga Virology. ,vol. 150, pp. 170- 177 ,(1986) , 10.1016/0042-6822(86)90276-X
Karishma L. Manzur, Ming-Ming Zhou, An archaeal SET domain protein exhibits distinct lysine methyltransferase activity towards DNA-associated protein MC1-α FEBS Letters. ,vol. 579, pp. 3859- 3865 ,(2005) , 10.1016/J.FEBSLET.2005.05.026
C. Qian, M. -M. Zhou, SET domain protein lysine methyltransferases: Structure, specificity and catalysis. Cellular and Molecular Life Sciences. ,vol. 63, pp. 2755- 2763 ,(2006) , 10.1007/S00018-006-6274-5
Francesca De Santa, Maria Grazia Totaro, Elena Prosperini, Samuele Notarbartolo, Giuseppe Testa, Gioacchino Natoli, The Histone H3 Lysine-27 Demethylase Jmjd3 Links Inflammation to Inhibition of Polycomb-Mediated Gene Silencing Cell. ,vol. 130, pp. 1083- 1094 ,(2007) , 10.1016/J.CELL.2007.08.019
Karl Agger, Paul A. C. Cloos, Jesper Christensen, Diego Pasini, Simon Rose, Juri Rappsilber, Irina Issaeva, Eli Canaani, Anna Elisabetta Salcini, Kristian Helin, UTX and JMJD3 are histone H3K27 demethylases involved in HOX gene regulation and development. Nature. ,vol. 449, pp. 731- 734 ,(2007) , 10.1038/NATURE06145
Ming Kang, Micheal Graves, Mario Mehmel, Anna Moroni, Sabrina Gazzarrini, Gerhard Thiel, James R Gurnon, James L Van Etten, Genetic diversity in chlorella viruses flanking kcv, a gene that encodes a potassium ion channel protein Virology. ,vol. 326, pp. 150- 159 ,(2004) , 10.1016/J.VIROL.2004.05.023
R. Cao, L. Wang, H. Wang, L. Xia, H. Erdjument-Bromage, P. Tempst, R. S. Jones, Y. Zhang, Role of Histone H3 Lysine 27 Methylation in Polycomb-Group Silencing Science. ,vol. 298, pp. 1039- 1043 ,(2002) , 10.1126/SCIENCE.1076997