Molecular characterization of thymidine kinase mutants of human cells induced by densely ionizing radiation.
作者： Amy Kronenberg , John B. Little
DOI: 10.1016/0027-5107(89)90004-3
关键词: Restriction fragment length polymorphism 、 Biology 、 Complementary DNA 、 Allele 、 Homologous chromosome 、 Restriction fragment 、 Genetics 、 Locus (genetics) 、 Molecular biology 、 Mutant 、 Thymidine kinase
摘要: Abstract In order to characterize the nature of mutants induced by densely ionizing radiations at an autosomal locus, we have isolated a series 99 thymidine kinase (tk) human TK6 lymphoblastoid cells irradiated with either fast neutrons or accelerated argon ions. Individual mutant clones were examined for alterations in their restriction fragment pattern after hybridization cDNA probe tk. A length polymorphism (RFLP) allowed identification active tk allele. Among neutron-induced mutants, 34 52 exhibited loss previously allele while 6 intragenic rearrangements. argon-induced 27 46 and 10 showed rearrangements within locus. The remaining had patterns indistinguishable from parent. Each was further structural c-erbA1 locus which has been localized chromosome 17q11-q22, some unknown distance 17q21-q22. substantial proportion (54%) radiation c-erg on homologous chromosome, suggesting that mutations involve large-scale genetic changes.
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