Radiation-induced base substitution mutagenesis in single-stranded DNA phage M13

摘要: Radiation-induced mutagenesis of Escherichia coli depends on the ability bacteria to perform error-prone repair1,2. This SOS repair activity is induced by irradiation and gives rise mutations both at site radiation-induced lesions (targeted mutations) in undamaged DNA (untargeted mutations)1,3–6. To elucidate relative contributions targeted untargeted γ UV radiation we have determined sequences 174 M13 revertant phages isolated from stocks irradiated or unirradiated amber mutants grown (SOS-induced) (non-induced) host bacteria. As reported here, differences spectra base change various conditions were apparent, but detected no obvious specificity mutagenesis. In particular, our conditions, pyrimidine dimers did not seem be principal sites UV-induced substitution mutagenesis, suggesting that such occurs other than dimers, untargeted.