Acclimation-induced changes in cell membrane composition and influence on cryotolerance of in vitro shoots of native plant species

摘要: Cell membranes are the primary sites of cryopreservation injury and measuring changes to membrane composition arising from cold acclimation may assist with providing a rationale for optimising methods. Shoot tips two south-west Western Australian species, Grevillea scapigera Loxocarya cinerea, Arabidopsis thaliana (reference species) were subjected using droplet vitrification protocol. Two pre-conditioning regimes involving constant temperature (23 °C, CT 12 h light/dark cycle) or an alternating (AT) regime (20/10 °C compared. Soluble sugars, sterols phospholipids present in shoot analysed. Use AT (acclimation) resulted modest decrease cryotolerance A. thaliana, increased G. scapigera, survival non-frozen control explants L. cinerea comparison pre-conditioning. Increased was accompanied by higher total sugar sterol phospholipid content, as well increase strong hydrating classes such phosphatidylcholine. The double bond index bound fatty acyl chains greater after pre-conditioning, mostly due amount monoenes trienes cinerea. These findings suggest that treatments vitro plants can have positive influence on some plant species this be related observed overall cell membranes. However, alternative factors (e.g. oxidative stress) equally important other cinerea).