Influence of abiotic stress preconditioning on antioxidant enzymes in shoot tips of Lomandra sonderi (Asparagaceae) prior to cryostorage
作者： B. Funnekotter , A. Sortey , E. Bunn , S. R. Turner , R. L. Mancera
DOI: 10.1071/BT16006
关键词: Biology 、 Antioxidant 、 Shoot 、 Micropropagation 、 Glutathione reductase 、 Botany 、 Superoxide dismutase 、 Cryopreservation 、 Glutathione peroxidase 、 Catalase
摘要: Lomandra sonderi (F.Muell.) Ewart (Asparagaceae) is endemic to the south-west Western Australian jarrah (Eucalyptus marginata Donn ex Sm.) forest region, and a difficult propagate species important post-mining restoration. Micropropagation only way currently produce plants of this for This study describes investigations into optimising cryopreservation efficient long-term germplasm storage. In order investigate effect preconditioning on post-cryogenic survival shoot tips, in vitro grown were exposed range light-, temperature- osmotic-induced treatments under culture room conditions 3 weeks. Room temperature (24°C) resulted greatest survival, followed by low light (17 µmol m–2 s–1) preconditioning. Alternating (25/5°C), high (35°C), sucrose (180 mM) (93 µmol m–2 s–1) all led significantly progressively lower tip than Antioxidant activity superoxide dismutase preconditioned tips showed positive correlation overall, whereas activities glutathione reductase, peroxidase catalase little correlation. Analysis throughout protocol that reductase decreased after cryopreservation, whilst did not change.
curtin.edu.au参考文章(39)
Y. P. S. Bajaj, Cryopreservation of Plant Cell, Tissue, and Organ Culture for the Conservation of Germplasm and Biodiversity Springer, Berlin, Heidelberg. pp. 3- 28 ,(1995) , 10.1007/978-3-662-03096-7_1
Barker Re, Reed Bm, Chang Y, Cold acclimation improves recovery of cryopreserved grass (Zoysia and Lolium sp.). Cryo letters. ,vol. 21, pp. 107- 116 ,(2000)
B. Vandenbussche, S. Leurdian, V. Verdoodt, M. Gysemberg, M. De Proft, Changes in sugar content and fatty acid composition of in vitro sugar beet shoots after cold acclimation : influence on survival after cryopreservation Plant Growth Regulation. ,vol. 28, pp. 157- 163 ,(1999) , 10.1023/A:1006262827160
Gaber Gomaa Shehab, Osama Konsswa AHMED, Hossam Saad El-Beltagi, None, Effects of Various Chemical Agents for Alleviation of Drought Stress in Rice Plants (Oryza sativa L.) Notulae Botanicae Horti Agrobotanici Cluj-napoca. ,vol. 38, pp. 139- 148 ,(2010) , 10.15835/NBHA3813627
Raquel Folgado, Bart Panis, Kjell Sergeant, Jenny Renaut, Rony Swennen, Jean-Francois Hausman, Unravelling the effect of sucrose and cold pretreatment on cryopreservation of potato through sugar analysis and proteomics. Cryobiology. ,vol. 71, pp. 432- 441 ,(2015) , 10.1016/J.CRYOBIOL.2015.09.006
Ricardo L Mancera, Bryn Funnekotter, Anja Kaczmarczyk, Eric Bunn, Akshay Menon, Shane Turner, Cryopreservation of Lomandra sonderi (Asparagaceae) shoot tips using droplet-vitrification. Cryo letters. ,vol. 33, pp. 259- 270 ,(2012)
Anja Kaczmarczyk, Natalia Shvachko, Yulia Lupysheva, Mohammad-Reza Hajirezaei, E. R. Joachim Keller, Influence of alternating temperature preculture on cryopreservation results for potato shoot tips Plant Cell Reports. ,vol. 27, pp. 1551- 1558 ,(2008) , 10.1007/S00299-008-0574-1
Bryn Funnekotter, Anja Kaczmarczyk, Shane R. Turner, Eric Bunn, Wenxu Zhou, Steven Smith, Gavin Flematti, Ricardo L. Mancera, Acclimation-induced changes in cell membrane composition and influence on cryotolerance of in vitro shoots of native plant species Plant Cell Tissue and Organ Culture. ,vol. 114, pp. 83- 96 ,(2013) , 10.1007/S11240-013-0308-0
John H. Crowe, Lois M. Crowe, John F. Carpenter, A.S. Rudolph, Christina Aurell Wistrom, B.J. Spargo, T.J. Anchordoguy, Interactions of sugars with membranes Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes. ,vol. 947, pp. 367- 384 ,(1988) , 10.1016/0304-4157(88)90015-9
Anja Kaczmarczyk, Shane R. Turner, Eric Bunn, Ricardo L. Mancera, Kingsley W. Dixon, Cryopreservation of threatened native Australian species—what have we learned and where to from here? In Vitro Cellular & Developmental Biology – Plant. ,vol. 47, pp. 17- 25 ,(2011) , 10.1007/S11627-010-9318-3