Molecular analysis of the rearranged genome and chimeric mRNAs caused by the t(6;11)(q27;q23) chromosome translocation involving MLL in an infant acute monocytic leukemia
作者： Yukihiro Akao , Masaharu Isobe
DOI: 10.1002/(SICI)1098-2264(200004)27:4<412::AID-GCC11>3.0.CO;2-X
关键词: Southern blot 、 Gene 、 Biology 、 Myeloid-Lymphoid Leukemia Protein 、 Molecular biology 、 Acute monocytic leukemia 、 Chromosomal translocation 、 Exon 、 Genetics 、 Chromosome 、 Complementary DNA
摘要: Chromosomal analysis of acute monocytic leukemia cells in a female infant revealed t(6;11)(q27;q23) translocation. Southern blot with cDNA probe the MLL gene at chromosome band 11q23 indicated that breakpoint was an 8.3-kb BamHI fragment contained exons 5-11 gene. Northern showed faint corresponding to chimeric transcript. Structural genomic clone rearranged from der(11) demonstrated be localized between 6 and 7 lie Alu sequence this region. The partner fused 3' part shown AF6 on 6q27 by situ hybridization nucleotide sequencing clones. However, it exon 5 one clone, whereas most clones were 6/AF6 These findings indicate is spliced out process transcription variant MLL/AF6. In addition, we able detect splicing either MLL/AF6 or transcripts untranslocated chromosomes reverse transcriptase-polymerase chain reaction. detailed genetic map determined radiation hybrid mapping.
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