Oxidative stress induced apoptosis of human lung carcinoma (A549) cells by a novel copper nanorod formulation.
作者： Menaka C. Thounaojam , Ravirajsinh N. Jadeja , Mayur Valodkar , Padamanabhi S. Nagar , Ranjitsinh V. Devkar
DOI: 10.1016/J.FCT.2011.07.055
关键词: Oxidative stress 、 Glutathione 、 Viability assay 、 Cytotoxicity 、 Molecular biology 、 Propidium iodide 、 A549 cell 、 Cancer cell 、 Apoptosis 、 Chemistry
摘要: This study elucidates the process of synthesis copper (Cu) nanorods using almond skin extract as stabilizing cum capping agent. These were (about 200 nm long and 40 wide) characterized by transmission electron microscopy (TEM). Further, cytotoxicity potential these was evaluated in A549 cells (Human lung carcinoma cell line) via viability assay extracellular lactate dehydrogenase (LDH) release. Also, reduced glutathione (GSH), lipid peroxidation (LPO), cellular oxidative stress (Rhodamine 123 florescence) apoptosis (Annexin V FITC/Propidium iodide staining) also investigated control treated cells. Results indicated that Cu induced apoptotic death cancer induction stress, depletion antioxidants mitochondrial dysfunction. reports a novel capped its an anticancer agent against
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