A 3D soil-like nanostructured fabric for the development of bacterial biofilms for agricultural and environmental uses

摘要: The present study aimed at creating a solid nanostructured scaffold suitable for the development of bacterial biofilms, and that enabled bacteria to successfully resist harsh nutritive environmental conditions potential applications. As model system, we combined self-standing electrospun poly(e-caprolactone)-based (EN-PCLS) with Burkholderia terricola cells. structure was fabricated on purpose include microbeads nanofibres, mimic 3D morphological spatial architecture soil microscale nanoscale, thus favouring biofilms. resulting framework displayed an extensive porosity pore interconnectivity, honeycomb wax cell-like arrangement reproducing large cavities in soil. first deposited conditioning film onto EN-PCLS facilitate adhesion. attachment preferentially observed nanofibres cells prevalently oriented along them, it persisted until late incubation under stirring. Notably, beads appeared bacteria-free if deprived them. Over time, interaction improved terms stability upon formation appendages release extracellular polymeric substances (EPS). more stable adhesion enhanced colonisation flat aggregations nanofibrous areas inside frames EN-PCLS. Also, micro- macrocolonies were formed, hung between walls. Such microbial organisation evolved into mature biofilm within 7 d incubation, where formed densely packed layers embedded coated EPS matrix covered as thick blanket whole soil-like At final stage (11 d), frameworks both partially degraded, latter being probably utilised C-source. Hence, proposed here has develop biofilms natural supports than traditional studies. Thus, this strategy seems promising scaffolds be used successful nanobiotechnological carriers applications agriculture (nutrient supply control plant diseases) environment (bioremediation polluted soils wastewater).