Synergistic anticancer effect of curcumin and chemotherapy regimen FP in human gastric cancer MGC‑803 cells

摘要: Curcumin is an anticancer compound that exerts anti-proliferative and apoptotic effects via multiple molecular targets. The purpose of the present study was to investigate curcumin in combination with 5-fluorouracil plus cisplatin (FP) on MGC-803 human gastric cancer cell line. Following treatment and/or FP for 24, 48 72 h, viability, cycle progression apoptosis rate were evaluated using MTT assay, flow cytometry dual acridine orange/ethidium bromide staining, respectively. In addition, colony formation, Transwell migration caspase-3/caspase-8 activity assays performed. expression regulator B-cell lymphoma-2 (Bcl-2) Bcl-2-associated X protein (Bax) detected by western blotting analysis. FP, formation significantly reduced compared untreated control group. apoptosis, Bax increased, whereas Bcl-2 following efficacy combined low-dose high-dose (P<0.05). Therefore, may enhance chemotherapy cells through promotion caspase-3/caspase-8, signaling pathways. These results suggest serve as a synergistic drug regimen cancer.