Rapid Detection of Subtype H10N8 Influenza Virus by One-Step Reverse-Transcription Loop-Mediated Isothermal Amplification Methods
作者： Hongmei Bao , Xiaoxiao Feng , Yong Ma , Jianzhong Shi , Yuhui Zhao
DOI: 10.1128/JCM.02165-15
关键词: Biology 、 Influenza A virus 、 Hemagglutinin (influenza) 、 Reverse Transcription Loop-mediated Isothermal Amplification 、 Neuraminidase 、 Virology 、 Virus 、 Nucleic acid amplification technique 、 Reverse transcriptase 、 Molecular biology 、 Loop-mediated isothermal amplification
摘要: We developed hemagglutinin- and neuraminidase-specific one-step reverse transcription-loop-mediated isothermal amplification assays for detecting the H10N8 virus. The detection limit of was 10 copies virus, did not amplify nonspecific RNA. can detect virus from chicken samples with high sensitivity specificity, they serve as an effective tool monitoring in live poultry markets.
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