Effects of a Finite-sized Pinhole on 3D Image Formation in Confocal Two-photon Fluorescence Microscopy

摘要: Abstract We investigate three-dimensional (3D) imaging properties in confocal two-photon (2-p) fluorescence microscopy with a finite-sized detector. The 3D intensity point spread function, the optical transfer function (OTF), strength of sectioning and signal levels for point, planar volume objects are calculated order to reveal their relationships detector size. It is shown that 2-p gives super-resolution, compared conventional without pinhole. Although can be attained latter case, its inferior case. In particular, result negative values OTF reduce effective cut-off spatial frequencies, thus degrading capability imaging. Unlike single-photon microscope, there no missing cone frequencies for...