Resolution in optical microscopy.
作者： James E.N. Jonkman , Jim Swoger , Holger Kress , Alexander Rohrbach , Ernst H.K. Stelzer
DOI: 10.1016/S0076-6879(03)60122-9
关键词:
摘要: Publisher Summary This chapter describes resolution in optical microscopy. Optical microscopes are fundamentally limited the they can achieve. The depends on wavelength of light (both incident and detected), numerical aperture (NA) arrangement, specimen to be observed or experiment performed. Live specimens also dynamic sensitive photobleaching thermal damage, which imposes a limit duration for power light. Fluorescence is excited throughout its illumination cone, but only fluorescence emitted from focal point imaged through confocal pinhole detector. A useful tool comparing performance point-spread function (PSF). PSF defined two complementary. intensity (hereafter referred simply as PSF) measured by taking images of—for example, subresolution bead it scanned focus microscope. Real are, course, rarely sources.
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