A specific immunological probe for the major myelin proteolipid
作者： E. Trifilieff , B. Luu , J.L. Nussbaum , G. Roussel , A.Espinosa de los Monteros
DOI: 10.1016/0014-5793(86)80412-4
关键词: Myelin proteolipid 、 Biochemistry 、 Membrane protein 、 Proteolipid protein 1 、 Antibody 、 Peptide 、 Myelin 、 Oligodendrocyte 、 Biology 、 Proteolipids 、 Biophysics 、 Genetics 、 Cell biology 、 Molecular biology 、 Structural biology
摘要: Major myelin proteolipid (MMPL, also called PLP) and DM-20 are the two major intrinsic membrane proteins of CNS myelin. A specific immunological probe was obtained for MMPL by raising antibodies against synthetic tridecapeptide 117–129 MMPL. Antibodies this peptide reacted with but dit not cross react DM-20, while both proteolipids had been shown previously to be recognized directed C-terminal hexapeptide This is in accordance previous findings showing that diners only from a deletion residues 100–140 (±few units). Furthermore, site-specific recognizes its native form oligodendrocytes primary glial cell cultures.
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