Macrophage Activation by a Lipophilic Derivative of Muramyldipeptide within Nanocapsules: Investigation of the Mechanism of Drug Delivery
作者： Isabelle Seyler , Martine Appel , Jean-Philippe Devissaguet , Philippe Legrand , Gillian Barratt
关键词: Biochemistry 、 Lipoprotein 、 Phagocytosis 、 Biophysics 、 Cholesterol 、 Low-density lipoprotein 、 Centrifugation 、 Nanocapsules 、 Chemistry 、 Cytochalasin B 、 Drug delivery
摘要: Different colloidal formulations: nanocapsules (NC), emulsion and micelles, containing the lipophilic immunomodulator muramyltripeptide cholesterol (MTP-Chol) induce nitric oxide synthase activity in RAW 264.7 cell line. The use of cytochalasin B, an inhibitor movements, showed that phagocytosis was important mechanism as far NC were concerned. However, when cells separated from particles by a membrane 100 nm pore size, significant could still be obtained, provided serum included medium. To determine whether low-density lipoprotein (LDL) might act intermediate carrier for MTP-Chol, transfer to LDL studied ultrafiltration/centrifugation method followed HPLC analysis. Although MTP-Chol transferred LDL, purified human added serum-free medium, activation reduced, rather than increased. This suggests intact carrying is not taken up great extent these macrophages.
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