Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR
作者： Takashi Watanabe , Tomohiro Miura , Yusuke Degawa , Yuna Fujita , Masaaki Inoue
关键词: Housekeeping gene 、 Candidate gene 、 Small-cell carcinoma 、 Gene expression profiling 、 DNA microarray 、 Biology 、 Large cell 、 Adenocarcinoma 、 Molecular biology 、 Lung cancer
摘要: Background: Lung cancers are the most common type of human malignancy and intractable. generally classified into four histopathological subtypes: adenocarcinoma (AD), squamous cell carcinoma (SQ), large (LC), small (SC). Molecular biological characterization these subtypes has been performed mainly using DNA microarrays. In this study, we compared gene expression profiles twelve lung cancer lines more reliable quantitative real-time PCR (qPCR). Results: We selected 100 genes from public microarray data examined them by analysis in eight test (A549, ABC-1, EBC-1, LK-2, LU65, LU99, STC 1, RERF-LC-MA) a normal control line (MRC-9). From this, extracted 19 candidate genes. quantified housekeeping gene, GAPDH, with qPCR, same plus additional validation (RERF-LC-MS, LC-1/sq, 86-2, MS-1-L). Finally, characterized principal component (PCA) profiling for 12 (AMY2A, CDH1, FOXG1, IGSF3, ISL1, MALL, PLAU, RAB25, S100P, SLCO4A1, STMN1, TGM2). The combined PCA pathway analyses suggested that were related to adhesion, growth, invasion. S100P AD cells CDH1 SQ identified as markers based on their upregulation results analysis. Immunohistochemistry RAB25 was closely correlated expression. Conclusions: These show subtypes, represented lines, well qPCR examined. Certain genes, particular may be especially important distinguishing different subtypes. Our confirm provide useful tool characterizing discuss possible clinical applications approach.
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