Fetal tendon wound size modulates wound gene expression and subsequent wound phenotype

摘要: The fetal response to small tendon injury results in regenerative or scarless healing and is characterized by a markedly diminished cellular inflammatory response, lack of fibroplasia, restoration normal tissue architecture. We hypothesized that an increasing wound size would lead increased inflammation change from reparative scar formation. created large wounds early gestation sheep used histology assess architecture, immunohistochemistry the ovine-specific gene microarrays, real-time reverse transcription-polymerase chain reaction measure expression injury. Small showed phenotype with orderly deposition collagen fibers while disorderly consistent had few cells at 7 28 days after injury, whereas significant cell infiltrate resolved days. At 3 days, differential genes involved were seen between wounds. By polymerase also significantly interleukin-6, interleukin-8, transforming growth factor-β1, factor-β3, compared Increasing proinflammatory expression, infiltration, healing. This model allows process be examined without confounding variable gestational age.