Molecular aspects of zygotic embryogenesis in sunflower (Helianthus annuus L.): correlation of positive histone marks with HaWUS expression and putative link HaWUS/HaL1L.

摘要: The link HaWUS/ HaL1L , the opposite transcriptional behavior, and decrease/increase in positive histone marks bond to both genes suggest an inhibitory effect of WUS on sunflower zygotic embryos. In Arabidopsis, a group of transcription factors implicated earliest events embryogenesis is WUSCHEL-RELATED HOMEOBOX (WOX) protein family including WUSCHEL (WUS) other 14 WOX protein, some which contain conserved WUS-box domain addition homeodomain. WUS transcripts appear very early embryogenesis, at 16-cell embryo stage, but gradually become restricted center developing shoot apical meristem (SAM) primordium continues be expressed cells niche/organizing SAM floral meristems maintain stem cell population. Moreover, has decisive roles embryonic program presumably promoting vegetative-to-embryonic transition and/or maintaining identity cells. However, data on direct interaction between key for seed development (as LEC1 L1L) are not collected. novelty this report consists characterization Helianthus annuus (HaWUS) gene its analysis regarding pattern methylated lysine 4 (K4) Histone H3 acetylated during development. Also, parallel investigation was performed HaL1L since two copies WUS-binding site (WUSATA), previously identified nucleotide sequence, were able bound by HaWUS recombinant suggesting described transcription.