Shadow enhancers enable Hunchback bifunctionality in the Drosophila embryo
作者： Max V. Staller , Ben J. Vincent , Meghan D. J. Bragdon , Tara Lydiard-Martin , Zeba Wunderlich
关键词: Embryo 、 DNA-binding protein 、 Cell biology 、 Enhancer trap 、 Transcription factor 、 Blastoderm 、 Drosophila Protein 、 Biology 、 Genetics 、 Enhancer 、 Enhancer RNAs
摘要: Hunchback (Hb) is a bifunctional transcription factor that activates and represses distinct enhancers. Here, we investigate the hypothesis Hb can activate repress same enhancer. Computational models predicted bifunctionally regulates even-skipped (eve) stripe 3+7 enhancer (eve3+7) in Drosophila blastoderm embryos. We measured modeled eve expression at cellular resolution under multiple genetic perturbations found eve3+7 could not explain endogenous 7 behavior. Instead, controlled by two enhancers: canonical sequence encompassing minimal 2 (eve2+7). 7, but it executes these activities on different pieces of regulatory DNA--it eve2+7 These "shadow enhancers" use logic to create pattern.
harvard.edu
sci-hub.se 参考文章(70)
K. Harding, T. Hoey, R. Warrior, M. Levine, Autoregulatory and gap gene response elements of the even-skipped promoter of Drosophila. The EMBO Journal. ,vol. 8, pp. 1205- 1212 ,(1989) , 10.1002/J.1460-2075.1989.TB03493.X
S. Small, A. Blair, M. Levine, Regulation of even-skipped stripe 2 in the Drosophila embryo. The EMBO Journal. ,vol. 11, pp. 4047- 4057 ,(1992) , 10.1002/J.1460-2075.1992.TB05498.X
Harold D. Kim, Tal Shay, Erin K. O’Shea, Aviv Regev, Transcriptional regulatory circuits: predicting numbers from alphabets. Science. ,vol. 325, pp. 429- 432 ,(2009) , 10.1126/SCIENCE.1171347
Bruno Di Stefano, Jose Luis Sardina, Chris van Oevelen, Samuel Collombet, Eric M. Kallin, Guillermo P. Vicent, Jun Lu, Denis Thieffry, Miguel Beato, Thomas Graf, C/EBPα poises B cells for rapid reprogramming into induced pluripotent stem cells Nature. ,vol. 506, pp. 235- 239 ,(2014) , 10.1038/NATURE12885
Nicolás Frankel, Gregory K. Davis, Diego Vargas, Shu Wang, François Payre, David L. Stern, Phenotypic robustness conferred by apparently redundant transcriptional enhancers Nature. ,vol. 466, pp. 490- 493 ,(2010) , 10.1038/NATURE09158
David S Latchman, Transcription factors: bound to activate or repress. Trends in Biochemical Sciences. ,vol. 26, pp. 211- 213 ,(2001) , 10.1016/S0968-0004(01)01812-6
Leslie Dunipace, Abbie Saunders, Hilary L. Ashe, Angelike Stathopoulos, Autoregulatory Feedback Controls Sequential Action of cis-Regulatory Modules at the brinker Locus Developmental Cell. ,vol. 26, pp. 536- 543 ,(2013) , 10.1016/J.DEVCEL.2013.08.010
Majid Kazemian, Charles Blatti, Adam Richards, Michael McCutchan, Noriko Wakabayashi-Ito, Ann S Hammonds, Susan E Celniker, Sudhir Kumar, Scot A Wolfe, Michael H Brodsky, Saurabh Sinha, None, Quantitative analysis of the Drosophila segmentation regulatory network using pattern generating potentials. PLOS Biology. ,vol. 8, pp. 51- 52 ,(2010) , 10.1371/JOURNAL.PBIO.1000456
Xin He, Md. Abul Hassan Samee, Charles Blatti, Saurabh Sinha, Thermodynamics-Based Models of Transcriptional Regulation by Enhancers: The Roles of Synergistic Activation, Cooperative Binding and Short-Range Repression PLOS Computational Biology. ,vol. 6, ,(2010) , 10.1371/JOURNAL.PCBI.1000935
Jin Jiang, Michael Levine, Binding affinities and cooperative interactions with bHLH activators delimit threshold responses to the dorsal gradient morphogen Cell. ,vol. 72, pp. 741- 752 ,(1993) , 10.1016/0092-8674(93)90402-C