Physiological Contribution of CD44 as a Ligand for E-Selectin during Inflammatory T-Cell Recruitment

摘要: Endothelial selectins guide the migration of inflammatory T cells to extralymphoid tissues. Whereas P-selectin glycoprotein ligand-1 (PSGL-1) functions as exclusive ligand for P-selectin, it acts in coordination with additional glycoproteins mediate E-selectin binding. CD44 can act one such neutrophils, but its contribution lymphocytes remains unexplored. We have used real-time vivo imaging cremasteric and dermal microcirculations explore dynamics leukocyte recruitment, well physiological a model Th1-driven inflammation. CD4+ T-cell rolling frequency kinetics, arrest, were dependent on endothelial markedly altered under conditions. extracted from Th1 bound soluble vitro cooperated PSGL-1 by controlling velocities promoting firm arrest. Using several competitive recruitment assays delayed-type hypersensitivity model, we show that combined absence impairs beyond alone. Differential expression fucosyltransferases these may account differential use ligands relative neutrophils. Our results identify mechanisms which modulates response.