Simple and facile preparation of recombinant human bone morphogenetic protein-2 immobilized titanium implant via initiated chemical vapor deposition technique to promote osteogenesis for bone tissue engineering application.

摘要: Over the past few decades, titanium (Ti) implants have been widely used to repair fractured bones. To promote osteogenesis, immobilization of osteoinductive agents, such as recombinant human bone morphogenic protein-2 (rhBMP2), onto Ti surface is required. In this study, we prepared rhBMP2 immobilized on glycidyl methacrylate (GMA) deposited through initiated chemical vapor deposition (iCVD) technique. After preparation, bio-functionalized was characterized by physicochemical analysis. For in vitro analysis, developed evaluated cell proliferation, alkaline phosphatase activity, calcium deposition, and real-time polymerase chain reaction verify their osteogenic activity against adipose-derived stem cells (hASCs). The GMA found effectively immobilize a large dose compared untreated Ti. Additionally, showed significantly enhanced differentiation increased with nontoxic viability. These results clearly confirm that our strategy may provide simple, solvent-free prepare an for tissue engineering applications.