Basal and apical regulation of VEGF-A and placenta growth factor in the RPE/choroid and primary RPE

摘要: PURPOSE Members of the vascular endothelial growth factor (VEGF) family are strongly involved in pathological processes retina, such as age-related macular degeneration and diabetic retinopathy. Cells retinal pigment epithelium (RPE) constitutively secrete VEGF-A, secretion placental (PlGF) has also been described. RPE cells polarized with different secretome at apical basal side. In this study, we evaluated regulation VEGF-A PlGF RPE/choroid explants primary cells. METHODS tissue were prepared from porcine eyes cultivated modified Ussing chambers, separating (choroid) supernatant. Primary on Transwell plates. Explants treated inhibitors for VEGFR-2 (SU1498), p38 (SB203580), transcription factors nuclear factor-kappa B (NF-κB) SP-1 (mithramycin), respectively. content was enzyme-linked immunosorbent assay (ELISA). addition, western blots performed. RESULTS RPE/choroid, can initially be found sides significantly more pronounced is differentially regulated sides, inhibition NF-κB showing strong effects apically basally after 24 h 48 h, displaying its effect mainly side some reducing VEGF only h. cell culture, similar found, or a decrease both an inhibitory contrast, not found. However, blot experiments exhibited significant protein under SU1498 treatment. organ cultures, site minute amounts apically. basally, while VEGFR2 to lesser degree displayed site. CONCLUSIONS secreted by complex, choroid. Although factors, polarity, secretion.