Functional Voltage‐Gated Calcium Channels Are Present in Human Embryonic Stem Cell‐Derived Retinal Pigment Epithelium
作者： Iina Korkka , Taina Viheriälä , Kati Juuti-Uusitalo , Hannele Uusitalo-Järvinen , Heli Skottman
DOI: 10.1002/SCTM.18-0026
关键词:
摘要: Retinal pigment epithelium (RPE) performs important functions for the maintenance of photoreceptors and vision. Malfunctions within RPE are implicated in several retinal diseases which transplantations stem cell-derived promising treatment options. Their success, however, is largely dependent on functionality transplanted cells. This requires correct cellular physiology, highly influenced by various ion channels RPE, including voltage-gated Ca2+ (CaV ) channels. study investigated localization CaV human embryonic cell (hESC)-derived RPE. Whole-cell patch-clamp recordings from these cells revealed slowly inactivating L-type currents comparable to freshly isolated mouse Some hESC-RPE also carried fast transient T-type resembling currents. These findings were confirmed immunostainings both hESC- that showed presence 1.2 1.3 as well 3.1 3.2. The major subtype, 1.3, changed during maturation co-localizing with pericentrin base primary cilium before reaching more homogeneous membrane Based functional assessment, participated regulation vascular endothelial growth factor secretion phagocytosis photoreceptor outer segments hESC-RPE. Overall, this demonstrates a machinery present mature hESC-RPE, success transplantation therapies. Stem Cells Translational Medicine 2019;8:179&15.
sci-hub.se 参考文章(86)
Bärbel Rohrer, Kannan Kunchithapautham, Andreas Genewsky, Olaf Strauß, Prolonged Src Kinase Activation, a Mechanism to Turn Transient, Sublytic Complement Activation into a Sustained Pathological Condition in Retinal Pigment Epithelium Cells Retinal Degenerative Diseases. ,vol. 801, pp. 221- 227 ,(2014) , 10.1007/978-1-4614-3209-8_29
Ram Kannan, Ning Zhang, Parameswaran G Sreekumar, Christine K Spee, Anthony Rodriguez, Ernesto Barron, David R Hinton, None, Stimulation of apical and basolateral VEGF-A and VEGF-C secretion by oxidative stress in polarized retinal pigment epithelial cells. Molecular Vision. ,vol. 12, pp. 1649- 1659 ,(2006)
Roy H. Steinberg, Yoshiki Ueda, Dihydropyridine-sensitive calcium currents in freshly isolated human and monkey retinal pigment epithelial cells. Investigative Ophthalmology & Visual Science. ,vol. 36, pp. 373- 380 ,(1995)
Rita Rosenthal, Hansjürgen Agostini, Gottfried Martin, Lutz Lothar Hansen, Olaf Strauss, Heinrich Heimann, Ca2+ channels in retinal pigment epithelial cells regulate vascular endothelial growth factor secretion rates in health and disease. Molecular Vision. ,vol. 13, pp. 443- 456 ,(2007)
Margrit Hollborn, Leon Kohen, Andreas Bringmann, Andreas Reichenbach, Peter Wiedemann, Stefanie Vogler, Regulation of the hyperosmotic induction of aquaporin 5 and VEGF in retinal pigment epithelial cells: involvement of NFAT5. Molecular Vision. ,vol. 21, pp. 360- 377 ,(2015)
Yao Li, Yi-Ting Tsai, Chun-Wei Hsu, Deniz Erol, Jin Yang, Wen-Hsuan Wu, Richard J. Davis, Dieter Egli, Stephen H. Tsang, Long-term Safety and Efficacy of Human-Induced Pluripotent Stem Cell (iPS) Grafts in a Preclinical Model of Retinitis Pigmentosa Molecular Medicine. ,vol. 18, pp. 1312- 1319 ,(2012) , 10.2119/MOLMED.2012.00242
Alexa Klettner, Johann Roider, Felix Treumer, Jens Lassen, Janina Flach, Leyla Kaya, Basal and apical regulation of VEGF-A and placenta growth factor in the RPE/choroid and primary RPE Molecular Vision. ,vol. 21, pp. 736- 748 ,(2015)
Nadine Reichhart, Magdalena Markowski, Shimpei Ishiyama, Andrea Wagner, Sergio Crespo-Garcia, Talitha Schorb, José S. Ramalho, Vladimir M. Milenkovic, Renate Föckler, Miguel C. Seabra, Olaf Strauß, Rab27a GTPase modulates L-type Ca2+ channel function via interaction with the II-III linker of CaV1.3 subunit Cellular Signalling. ,vol. 27, pp. 2231- 2240 ,(2015) , 10.1016/J.CELLSIG.2015.07.023
Deborah A. Ferrington, Debasish Sinha, Kai Kaarniranta, Defects in retinal pigment epithelial cell proteolysis and the pathology associated with age-related macular degeneration. Progress in Retinal and Eye Research. ,vol. 51, pp. 69- 89 ,(2016) , 10.1016/J.PRETEYERES.2015.09.002
Andreas Gal, Yun Li, Debra A. Thompson, Jessica Weir, Ulrike Orth, Samuel G. Jacobson, Eckart Apfelstedt-Sylla, Douglas Vollrath, Mutations in MERTK, the human orthologue of the RCS rat retinal dystrophy gene, cause retinitis pigmentosa. Nature Genetics. ,vol. 26, pp. 270- 271 ,(2000) , 10.1038/81555