Modulation of L-phenylalanine ammonia-lyase by pathway intermediates in cell suspension cultures of dwarf french bean (Phaseolus vulgaris L.).
作者： R. A. Dixon , T. Browne , M. Ward
DOI: 10.1007/BF00384656
关键词: Biochemistry 、 Phenylpropanoid 、 Enzyme assay 、 Enzyme 、 Cycloheximide 、 Phenylalanine ammonia-lyase 、 Cinnamic acid 、 Biology 、 Phenylalanine 、 Ferulic acid
摘要: The increase in extractable phenylalanine ammonia-lyase (PAL;EC 4.3.1.5.) activity induced French bean cell suspension cultures response to treatment with autoclaved ribonuclease A was inhibited by addition of the phenylpropanoid pathway intermediates cinnamic acid, 4-coumaric acid or ferulic acid. effectiveness inhibition order acid>4-coumaric acid>ferulic Cinnamic also PAL dilution suspensions into an excess fresh culture medium. Addition low concentrations (<10-5M) at time application gave variable responses ranging from 30–40% stimulation measured 8 h. Following 4–5 h after treatment, rapid increases followed equally declines were observed. acid-stimulated enzyme unaffected cycloheximide a concentration which complete ribonuclease-induced response. However, completely abolished subsequent decline activity. Treatment α-aminooxy-β-phenylpropionic (AOPPA) resulted increased but delayed rates appearance when compared controls not treated analogue. results are discussed relation current views on regulation levels higher plants.
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sci-hub.se 参考文章(28)
N. Amrhein, NOVEL INHIBITORS OF PHENYLPROPANOID METABOLISM IN HIGHER PLANTS Regulation of Secondary Product and Plant Hormone Metabolism#R##N#FEBS Federation of European Biochemical Societies: 12th Meeting, Dresden, 1978. pp. 173- 182 ,(1979) , 10.1016/B978-0-08-023179-2.50020-4
G. Engelsma, INHIBITION OF PHENYLALANINE AMMONIA-LYASE BY CINNAMIC ACID DERIVATIVES Regulation of Secondary Product and Plant Hormone Metabolism#R##N#FEBS Federation of European Biochemical Societies: 12th Meeting, Dresden, 1978. pp. 163- 172 ,(1979) , 10.1016/B978-0-08-023179-2.50019-8
J. Leggett Bailey, Techniques in protein chemistry Elsevier Pub. Co.. ,(1967)
Brigitte Betz, Eberhard Schäfer, Klaus Hahlbrock, Light-induced phenylalanine ammonia-lyase in cell-suspension cultures of Petroselinum hortense. Quantitative comparison of rates of synthesis and degradation. Archives of Biochemistry and Biophysics. ,vol. 190, pp. 126- 135 ,(1978) , 10.1016/0003-9861(78)90259-X
Klaus Hahlbrock, Hermann Ragg, Light-induced changes of enzyme activities in parsley cell suspension cultures Archives of Biochemistry and Biophysics. ,vol. 166, pp. 41- 46 ,(1975) , 10.1016/0003-9861(75)90362-8
Terry H. Attridge, Christopher B. Johnson, Harry Smith, Density-labelling evidence for the phytochrome-mediated activation of phenylalanine ammonia-lyase in mustard cotyledons. Biochimica et Biophysica Acta. ,vol. 343, pp. 440- 451 ,(1974) , 10.1016/0304-4165(74)90261-X
R.A. Dixon, D.S. Bendall, Changes in the levels of enzymes of phenylpropanoid and flavonoid synthesis during phaseollin production in cell suspension cultures of Phaseolus vulgaris Physiologial Plant Pathology. ,vol. 13, pp. 295- 306 ,(1978) , 10.1016/0048-4059(78)90046-2
R.A. Dixon, K.W. Fuller, Effects of synthetic auxin levels on phaseollin production and phenylalanine ammonia-lyase (PAL) activity in tissue cultures of Phaseolus vulgaris L Physiologial Plant Pathology. ,vol. 9, pp. 299- 312 ,(1976) , 10.1016/0048-4059(76)90063-1
C. J. Lamb, P. H. Rubery, Phenylalanine ammonia-lyase and cinnamic acid 4-hydroxylase: Product repression of the level of enzyme activity in potato tuber discs Planta. ,vol. 130, pp. 283- 290 ,(1976) , 10.1007/BF00387834
Daniel McMahon, Cycloheximide Is Not a Specific Inhibitor of Protein Synthesis in Vivo Plant Physiology. ,vol. 55, pp. 815- 821 ,(1975) , 10.1104/PP.55.5.815