Inhibition of mRNA levels and activities by trans-cinnamic acid in elicitor-induced bean cells

摘要: Abstract Addition of trans -cinnamic acid to bean cell suspension cultures after their treatment with fungal elicitor resulted in the loss induced phenylalanine ammonia-lyase (PAL) enzyme activity. In contrast elicitor-induced chalcone synthase (CHS) activity was arrested but did not decline, whereas isomerase (CHI) relatively unaffected. However, translational activities extracted polysomal mRNAs encoding these three enzymes were depressed by cinnamate treatments as levels PAL and CHS rates transcription genes measured isolated nuclei. Treatment l -α-aminooxy-phenylpropionic (AOPP), a potent inhibitor (and therefore production) vivo , increased production mRNAs. elicitor-treated inhibited appearance number polypeptides translated vitro from mRNA, although at least nine specifically treatment. We conclude that cinnamic potentially could act an modulator synthesis phenylpropanoid pathway it is yet fully possible rule out less specific inhibitory effects.