Isolation and characterization of polycyclic aromatic hydrocarbon-degrading Mycobacterium isolates from soil.
作者： C.D. Miller , K. Hall , Y.N. Liang , K. Nieman , D. Sorensen
DOI: 10.1007/S00248-003-1044-5
关键词: Mycobacterium 、 Pyrene 、 Biology 、 Botany 、 Polycyclic aromatic hydrocarbon 、 Microbiology 、 Bioremediation 、 Bacteria 、 Soil microbiology 、 Mycobacterium vanbaalenii 、 Ribosomal DNA
摘要: Bioremediation of soils contaminated with wood preservatives containing polycyclic aromatic hydrocarbons (PAHs) is desired because their toxic, mutagenic, and carcinogenic properties. Creosote preservative-contaminated at the Champion International Superfund Site in Libby, Montana currently undergo bioremediation a prepared-bed land treatment unit (LTU) process. Microbes isolated from these LTU rapidly mineralized (14)C-labeled PAH pyrene soil. Gram staining, electron microscopy, 16S rDNA-sequencing revealed that three bacteria, JLS, KMS, MCS, were Mycobacterium strains. The phylogeny rDNA showed they distinct other isolates PAH-degrading activities. Catalase superoxide dismutase (SOD) isozyme profiles confirmed each isolate was mycobacterium, vanbaalenii sp. nov, petroleum-contaminated We find dioxygenase genes nidA nidB are present Libby adjacent to sequence nidB-nidA, an order unique mycobacteria.
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