Phylogenetic comparison of two polycyclic aromatic hydrocarbon-degrading mycobacteria.

作者: M Govindaswami , D J Feldhake , B K Kinkle , D P Mindell , J C Loper

DOI: 10.1128/AEM.61.9.3221-3226.1995

关键词:

摘要: Two mycobacterial strains previously isolated from fossil-fuel-contaminated environments and shown to degrade four- and/or five-ring polycyclic aromatic hydrocarbons were further characterized. The two strains, PYR-I RJGII-135, had similar growth characteristics, colony morphologies, scotochromogenic pigmentations. DNA amplification fingerprints obtained with total genomic indicated some strain similarities but several distinctly different bands. Moreover, phylogenetic analysis based upon essentially full-length 16S rRNA gene sequences separates the as distinct species within fast-growing group of mycobacteria. Although both are thermosensitive, has bulged U between positions 184 193 characteristic thermotolerant Both potential use for reintroduction into bioremediation hydrocarbon-contaminated soils.

参考文章(22)
Hohzoh Kiyohara, Kazutaka Nagao, Keiji Yana, Rapid Screen for Bacteria Degrading Water-Insoluble, Solid Hydrocarbons on Agar Plates Applied and Environmental Microbiology. ,vol. 43, pp. 454- 457 ,(1982) , 10.1128/AEM.43.2.454-457.1982
R N Husson, B E James, R A Young, Gene replacement and expression of foreign DNA in mycobacteria. Journal of Bacteriology. ,vol. 172, pp. 519- 524 ,(1990) , 10.1128/JB.172.2.519-524.1990
M Govindaswami, T M Schmidt, D C White, J C Loper, Phylogenetic analysis of a bacterial aerobic degrader of azo dyes. Journal of Bacteriology. ,vol. 175, pp. 6062- 6066 ,(1993) , 10.1128/JB.175.18.6062-6066.1993
B Boldrin, A Tiehm, C Fritzsche, Degradation of phenanthrene, fluorene, fluoranthene, and pyrene by a Mycobacterium sp Applied and Environmental Microbiology. ,vol. 59, pp. 1927- 1930 ,(1993) , 10.1128/AEM.59.6.1927-1930.1993
C Fritzsche, Degradation of pyrene at low defined oxygen concentrations by a Mycobacterium sp. Applied and Environmental Microbiology. ,vol. 60, pp. 1687- 1689 ,(1994) , 10.1128/AEM.60.5.1687-1689.1994
M A Heitkamp, C E Cerniglia, Mineralization of polycyclic aromatic hydrocarbons by a bacterium isolated from sediment below an oil field. Applied and Environmental Microbiology. ,vol. 54, pp. 1612- 1614 ,(1988) , 10.1128/AEM.54.6.1612-1614.1988
A Fekete, J A Bantle, S M Halling, R W Stich, Amplification fragment length polymorphism in Brucella strains by use of polymerase chain reaction with arbitrary primers. Journal of Bacteriology. ,vol. 174, pp. 7778- 7783 ,(1992) , 10.1128/JB.174.23.7778-7783.1992
Gustavo Caetano-Anollés, Bassam Brant J., Gresshoff Peter M., DNA amplification fingerprinting using very short arbitrary oligonucleotide primers. Nature Biotechnology. ,vol. 9, pp. 553- 557 ,(1991) , 10.1038/NBT0691-553