Production of biohydrogen from sugars and lignocellulosic biomass using Thermoanaerobacter GHL15

摘要: Abstract The effect of culture parameters on hydrogen production using strain GHL15 in batch was investigated. belongs to the genus Thermoanaerobacter with 98.9% similarity yonseiensis and 98.5% keratinophilus a temperature optimum 65–70 °C pH 6–7. metabolizes various pentoses, hexoses, disaccharides acetate, ethanol, hydrogen, carbon dioxide. However substrate inhibition observed above 10 mM glucose concentration. Maximum yields were 3.1 mol H2 mol−1 at very low partial pressure hydrogen. Hydrogen from lignocellulosic biomass hydrolysates investigated culture. Various pretreatment methods examined including acid, base, enzymatic (Celluclast® Novozyme 188) hydrolysis. (5.8–6.0 mmol H2 g−1 dw) Whatman paper (cellulose) although less produced by other materials (maximally 4.83 mmol H2 g−1 dw grass hydrolysate). all improved acid alkaline pretreatments, maximum grass, 7.6 mmol H2 g−1 dw.