Effect of organic loading rate on hydrogen production from sugarcane vinasse in thermophilic acidogenic packed bed reactors
作者： Antônio Djalma Nunes Ferraz Júnior , Jorge Wenzel , Claudia Etchebehere , Marcelo Zaiat , None
DOI: 10.1016/J.IJHYDENE.2014.08.017
关键词:
摘要: Abstract This study evaluated the effect of organic loading rate (OLR) on hydrogen production in up-flow anaerobic packed bed reactors (APBR) continuously fed with sugarcane vinasse. Four thermophilic APBR were operated parallel at different ORL. Continuous was detected. The optimum OLR 84.2 kg-COD m−3 d−1 assessed by polynomial adjustment, which predicted a maximum Volumetric Hydrogen Production (VHP) and yield ( Y H 2 ) 1117.2 mL-H2 d−1 L−1reactor 2.4 mol-H2 mol−1total carbohydrates, respectively. microbial composition monitored using 16S rRNA gene Terminal Restriction Fragment Length Polymorphism (T-RFLP) analysis quantification Fe-hydrogenase real-time PCR affected OLR. number genes proportional to yield. Hydrogen-producing strains isolated, sequences highly homologous those Thermoanaerobacterium thermosaccarolyticum. ability vinasse as substrate for confirmed both strains.
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