Qualitative and quantitative analysis of active flavonoids in Flos Lonicerae by capillary zone electrophoresis coupled with solid‐phase extraction
作者： Jun Chen , Song-Lin Li , Ping Li , Yue Song , Xing-Yun Chai
关键词: Detection limit 、 Solid phase extraction 、 Chromatography 、 Sample preparation 、 Capillary electrophoresis 、 Quantitative analysis (chemistry) 、 Flos 、 Extraction (chemistry) 、 Avicularin 、 Chemistry
摘要: Flavonoids are an important bioactive group in the commonly used herbal medicine Flos Lonicerae. A new method of capillary zone electrophoresis (CZE) coupled with solid-phase extraction (SPE) was developed for simultaneous assay flavonoid aglycones and glycosides Optimum CZE separation achieved a background electrolyte (BGE) solution consisting 80 mM boric acid 20 phosphate acid, adjusted to pH 8.1, 15% acetonitrile (v/v) added, applying voltage 28 kV. The SPE pretreating complex matrix botanical materials good reproducibility obtained when avicularin as internal standard. Linearity excellent correlation coefficients (r2) range 0.9995-0.9999 detection limits were lower than 0.6 microg/mL four flavonoids. recoveries varied between 93 104% while relative standard deviations (RSDs) below 4.4% (n=3). successfully eight flavonoids quantification five species
sci-hub.se 参考文章(36)
Quantification of the flavonoid glycosides in Passiflora incarnata by capillary electrophoresis. Planta Medica. ,vol. 69, pp. 452- 456 ,(2003) , 10.1055/S-2003-39699
Lester Packer, Helmut Sies, Flavonoids and other polyphenols Academic Press. ,(2001)
Maxim Khrolenko, Paweł Dżygiel, Piotr Wieczorek, Combination of supported liquid membrane and solid-phase extraction for sample pretreatment of triazine herbicides in juice prior to capillary electrophoresis determination. Journal of Chromatography A. ,vol. 975, pp. 219- 227 ,(2002) , 10.1016/S0021-9673(02)01326-2
Xiuhua Sun, Changlu Gao, Weidong Cao, Xiurong Yang, Erkang Wang, Capillary electrophoresis with amperometric detection of curcumin in Chinese herbal medicine pretreated by solid-phase extraction Journal of Chromatography A. ,vol. 962, pp. 117- 125 ,(2002) , 10.1016/S0021-9673(02)00509-5
Alberto Escarpa, Marı́a Dolores Morales, Marı́a Cristina González, Analytical performance of commercially available and unavailable phenolic compounds using real samples by high-performance liquid chromatography–diode-array detection Analytica Chimica Acta. ,vol. 460, pp. 61- 72 ,(2002) , 10.1016/S0003-2670(02)00140-X
Tao Bo, Xuedong Yang, Fei Gao, Huwei Liu, Ke An Li, Lizhen Xiu, Optimized Separation of Pharmacologically Active Xanthones from Securidaca inappendiculata by Capillary Electrophoresis Chromatographia. ,vol. 55, pp. 217- 223 ,(2002) , 10.1007/BF02492145
B.X Mayer, How to increase precision in capillary electrophoresis. Journal of Chromatography A. ,vol. 907, pp. 21- 37 ,(2001) , 10.1016/S0021-9673(00)01057-8
Lorenzo Bramati, Francesca Aquilano, Piergiorgio Pietta, Unfermented rooibos tea: quantitative characterization of flavonoids by HPLC-UV and determination of the total antioxidant activity. Journal of Agricultural and Food Chemistry. ,vol. 51, pp. 7472- 7474 ,(2003) , 10.1021/JF0347721
Chris O. Okunji, Tantalia A. Ware, Rickey P. Hicks, Maurice M. Iwu, David J. Skanchy, Capillary Electrophoresis Determination of Biflavanones from Garcinia kola in Three Traditional African Medicinal Formulations Planta Medica. ,vol. 68, pp. 440- 444 ,(2002) , 10.1055/S-2002-32091
K.C.B De Souza, E.E.S Schapoval, V.L Bassani, LC determination of flavonoids: separation of quercetin, luteolin and 3-O-methylquercetin in Achyrocline satureioides preparations. Journal of Pharmaceutical and Biomedical Analysis. ,vol. 28, pp. 771- 777 ,(2002) , 10.1016/S0731-7085(01)00693-8