Molecular authentication of multi-species honeysuckle tablets.

摘要: Authenticating multi-species original raw materials in commercial formulations is difficult. Jin Yin Hua and Shan Hua, both classified as honeysuckle the Chinese Pharmacopoeia, are used various medicines. Differentiating one variety from another difficult based on chemical analysis. We developed molecular authentication of 3 brands tablets using allele-specific PCR. All contained Hua. also built a PCR-enzyme digestion method enzymatic mutation detection PCR fragments psbA-trnH trnL-trnF, restriction endonucleases HinfI NlaIV, respectively. The produced same result Sequence phylogenetic analyses show that YXC YQJ Lonicera japonica L. macranthoides materials, LYG japonica, hypoglauca, macranthoides.