Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers

摘要: A label-free, homogenous and sensitive one-step method for the molecular authentication of medicinal snakes has been developed by combining a rapid PCR technique with water-soluble cationic conjugated polyelectrolytes (CCPs). Three snake materials (Deinagkistrodon acutus, Zaocys dhumnades Bungarus multicinctus; total 35 specimens) 48 specimens similar morphologies textures were clearly distinguished naked eye utilizing CCP-based assay in high-throughput manner. The identification patented Chinese drugs was successfully performed using this detection system. In contrast to previous fluorescence-labeled oligonucleotide direct DNA stain hybridization assays, does not require designing dye-labeled primers, unfavorable dimer fluorescence is avoided method.