Barcoding Melting Curve Analysis for Rapid, Sensitive, and Discriminating Authentication of Saffron (Crocus sativus L.) from Its Adulterants
作者： Chao Jiang , Liang Cao , Yuan Yuan , Min Chen , Yan Jin
DOI: 10.1155/2014/809037
关键词: Botany 、 Crocus sativus 、 Calendula officinalis 、 Officinalis 、 Biology 、 Melting curve analysis 、 Daucus carota 、 Hemerocallis fulva 、 Crocus 、 Carthamus
摘要: Saffron (Crocus sativus L.) is one of the most important and expensive medicinal spice products in world. Because its high market value premium price, saffron often adulterated through incorporation other materials, such as Carthamus tinctorius L. Calendula officinalis flowers, Hemerocallis petals, Daucus carota fleshy root, Curcuma longa rhizomes, Zea may L., Nelumbo nucifera Gaertn. stigmas. To develop a straightforward, nonsequencing method for rapid, sensitive, discriminating detection these adulterants traded saffron, we report here application barcoding melting curve analysis (Bar-MCA) that uses universal chloroplast plant DNA region trnH-psbA to identify adulterants. When amplified at concentrations annealing temperatures optimized analysis, peaks were formed specific locations (81.92°C) adulterants: D. (81.60°C), C. (80.10°C), (79.92°C), Dendranthema morifolium (Ramat.) Tzvel. (79.62°C), N. (80.58°C), fulva (L.) (84.78°C), Z. mays (84.33°C). The constructed curves have significantly different peak or shapes. In conclusion, Bar-MCA could be faster more cost-effective authenticate detect
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