Assessing bioavailability of DDT and metabolites in marine sediments using solid-phase microextraction with performance reference compounds

摘要: Solid-phase microextraction (SPME) has often been used to estimate the freely dissolved concentration (Cfree) of organic contaminants in sediments. A significant limitation application SPME for Cfree measurement is requirement attaining equilibrium partition, which difficult strongly hydrophobic compounds such as DDT. method was developed using with stable isotope-labeled analogues performance reference (PRCs) measure DDT and metabolites (DDTs) marine Six 13C-labeled or deuterated PRCs were impregnated into polydimethylsiloxane (PDMS) fiber before use. Desorption from PDMS fibers absorption DDTs sediment isotropic a range sediments evaluated ex situ under well-mixed conditions. When applied historically contaminated Superfund site, PRC-SPME yielded values identical those found by conventional approach (Eq-SPME), wherease time mixing reduced 9 d only 9 h. The further against bioaccumulation Neanthes arenaceodentata without amendment activated carbon sand. Strong correlations consistently between derived concentrations on lipid-normalized tissue residues worms. Results present study clearly demonstrated feasibility coupling sampling greatly shorten time, thus affording much improved flexibility use bioavailability evaluation. Environ Toxicol Chem 2013;32:1946–1953. © 2013 SETAC