Trophic transfer and effects of DDT in male hornyhead turbot (Pleuronichthys verticalis) from Palos Verdes Superfund site, CA (USA) and comparisons to field monitoring

摘要: High concentrations of DDT and metabolites (ΣDDT) have been detected in sediment the demersal flatfish hornyhead turbot (Pleuronichtys verticalis) collected from Palos Verdes (PV), California, USA, a site contaminated with over 100 metric tons throughout 1960s-70s. This study was conducted to assess transfer ΣDDT PV-sediment into polychaetes (Neanthes arenaceodentata) turbot, investigate if responses turbots two different laboratory exposures mimic those caught PV (PV-turbot). Turbot fed PV-sediment-contaminated polychaete for 7 days had liver similar PV-turbot. After 28 days, also accumulated livers gavaged mixture. In vitro cell bioassays indicated significant increases 17β-estradiol equivalents (EEQ) bile extracts as compared control 7-day study. These corresponded measured PV-fish. Glucocorticoid receptor (GR), anti-androgen (anti-AR), estrogen (ER) or aryl hydrocarbon (AhR) activities were observed PV-sediment, PV-sediment-exposed worm. Anti-AR, AhR GR significantly higher than reference (San Diego, SD). Higher transcripts hepatic VTG, ERα ERβ found PV-turbot SD-turbot, but unaltered fish exposed sediment-contaminated worms In contrast, 28-day treatment showed lower EEQ VTG relative data that trophic sediment-associated field measurements residues in vitro ER bioactivities, failed in vivo biological effects fish. alone mimicked DDTs fish, did not correspond bioactivities.