Isolation of rat hepatocyte plasma membrane sheets and plasma membrane domains.

作者: Pamela L. Tuma , Ann L. Hubbard

DOI: 10.1002/0471143030.CB0302S02

关键词: HepatocytePlasmaALKALINE PHOSPHODIESTERASE IBiochemistryMarker enzymesBiologyNucleotidaseRat liverIndirect immunofluorescenceMembrane

摘要: This unit describes a method for isolation of plasma membrane sheets from rat liver. It also includes protocols preparation domains isolated and indirect immunofluorescence localization marker proteins associated with sheets. The has been updated assays the enzymes alkaline phosphodiesterase I, 5' nucleotidase, K+-stimulated.

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