作者: Pamela L. Tuma , Ann L. Hubbard
DOI: 10.1002/0471143030.CB0302S02
关键词: Hepatocyte 、 Plasma 、 ALKALINE PHOSPHODIESTERASE I 、 Biochemistry 、 Marker enzymes 、 Biology 、 Nucleotidase 、 Rat liver 、 Indirect immunofluorescence 、 Membrane
摘要: This unit describes a method for isolation of plasma membrane sheets from rat liver. It also includes protocols preparation domains isolated and indirect immunofluorescence localization marker proteins associated with sheets. The has been updated assays the enzymes alkaline phosphodiesterase I, 5' nucleotidase, K+-stimulated.