Genomic concatemerization/deletion in rotaviruses: a new mechanism for generating rapid genetic change of potential epidemiological importance.

摘要: Three variants of group A rotavirus with large changes in their gene 5 structures have been analyzed at the molecular level. The first these, P9 delta 5, was obtained during plaque purification undertaken as part biological cloning a field isolate virus. homolog this migrated just ahead normal segment 6 RNA, giving an estimated size 1,300 bp. Molecular and sequencing revealed it to single 308-bp deletion center sequence extending between nucleotides 460 768 sequence. This caused frameshift such that stop codon encountered 8 amino acids downstream point, predicted for protein product 150 compared 490 its normal-size counterpart. Attempts detect shortened virus-infected cells were not successful, indicating much less stable than full-length and/or had suffered change antigenicity. second two variants, brvA brvE, generated earlier study following high-multiplicity passage UKtc strain bovine rotavirus. Polyacrylamide gel electrophoresis analysis these nondefective showed approximately equal RNA 2 (approximately 2,700 bp) brvE 2,300 Both products, mimicking failing produce detectable whereas produced new virus-specific 80 kDa size. Full-length cDNA clones isolated, structure head-to-tail concatemerization copy concatemer covering 1 808 92 1579, total length 2,296 Sequencing across junction region copies they joined frame give combined open reading 728 amino-terminal consisting 258 fused carboxy terminus 21 490.(ABSTRACT TRUNCATED AT 400 WORDS)