Monoclonal antibody to single-stranded DNA is a specific and sensitive cellular marker of apoptosis.

摘要: The most widely used histochemical marker of apoptosis (in situend labeling, TUNEL) detects both apoptotic and necrotic cells evaluates only late stages apoptosis. Hence, a specific sensitive cellular is needed to determine the role death in biology pathology. present study describes novel immunohistochemical procedure for staining using monoclonal antibody (MAb) single-stranded DNA. This MAb stained all with morphology typical etoposide-treated HL-60, MOLT-4, R9 cell cultures, which was accompanied by high, moderate, low levels internucleosomal DNA fragmentation, respectively. TUNEL HL-60 nearly 60% MOLT-4 14% cultures. Apoptotic cells, progressed into secondary necrosis, retained became TUNEL-positive. Necrotic cultures treated sodium azide were TUNEL, but negative staining. All floating at stage MDA-MB-468 cisplatin TUNEL. However, among adherent early apoptosis, 20 times more than In histological sections human tumor xenografts, detected clusters viable tissue, did not stain areas central ischemic necrosis. contrast, nuclei areas. Thus, differentiates between necrosis