Glucose Depletion Rapidly Inhibits Translation Initiation in Yeast

摘要: Glucose performs key functions as a signaling molecule in the yeast Saccharomyces cerevisiae. depletion is known to regulate gene expression via pathways that lead derepression of genes at transcriptional level. In this study, we have investigated effect glucose on protein synthesis. We discovered withdrawal from growth medium led rapid inhibition synthesis and was readily reversed upon readdition glucose. Neither nor reactivation translation required new transcription. This also did not require activation amino acid starvation pathway or inactivation TOR kinase pathway. However, mutants repression (reg1, glc7, hxk2, ssn6), hexose transporter induction (snf3 rgt2), cAMP-dependent (tpk1(w) tpk2(w)) were resistant inhibitory effects translation. These findings highlight intimate connection between nutrient status cell its translational capacity. They help define area posttranscriptional regulation yeast.