Overexpression of the β-catenin binding domain of cadherin selectively kills colorectal cancer cells

摘要: The β-catenin pathway is involved in growth, differentiation and tumor formation. Suppression of activity by expressed inhibitors can cause growth arrest or apoptosis certain colon carcinoma lines. We compare the effects 2 inhibitors, a VE-cadherin cytoplasmic domain fragment (Cad5CD) truncated, dominant-negative Tcf4 (TcfDN), using microplate assay for cell death microarray gene expression analysis. cell-lethal shows that Cad5CD, when HT29 human cells 3 non-colon lines, selectively kills cells. Cad5CD overexpression inhibits β-catenin/Tcf4 transcriptional activity, as determined results from experiments. Our support view an attractive anti-cancer target, especially if binding site itself be exploited drug development. In addition, therapeutically relevant phenotypes such selectivity may difficult to predict analysis alone. Other more specialized phenotypic tests assays required. © 2003 Wiley-Liss, Inc.