Inhibition of xenograft human glioma tumor growth by lentivirus-mediated gene transfer of alphastatin
作者： HONGMIN CHE , JIANRONG SONG , SHIWEN GUO , WEIWEN WANG , GUODONG GAO
DOI: 10.3892/OR.2012.2187
关键词:
摘要: Angiogenesis is crucial for the development and metastasis of human brain glioma. Based on our previous successful construction a lentivirus-mediated alphastatin (an endogenous angiogenesis inhibitor) gene transfer system findings that exhibited potent inhibitory effects migration differentiation umbilical vein endothelial cell lines (HUVECs) induced by vascular growth factor (VEGF) or basic fibroblast (bFGF) in vitro, here, we investigated effect using lentiviral vectors to overexpress in glioma cells show whether sustained long-term expression diminishes tumor xenograft model. We found transduced sustainedly secreted alphastatin, which did not affect proliferative ability cells. Furthermore, xenografts treated with recombinant lentivirus were significantly smaller compared control vascularity within tumors was evidently decreased. Our data suggest stable inhibits inhibiting angiogenesis, probable mechanism suppressing turnover VE-cadherin membrane molecules.
sci-hub.se 参考文章(41)
Jen-Chine Wu, Horng-Chin Yan, Wei-Teing Chen, Wei-Hwa Chen, Chia-Jen Wang, Ying-Chih Chi, Woei-Yau Kao, JNK signaling pathway is required for bFGF-mediated surface cadherin downregulation on HUVEC. Experimental Cell Research. ,vol. 314, pp. 421- 429 ,(2008) , 10.1016/J.YEXCR.2007.10.002
T WRIGHT, L LEACH, P SHAW, P JONES, Dynamics of Vascular Endothelial-Cadherin and β-Catenin Localization by Vascular Endothelial Growth Factor-Induced Angiogenesis in Human Umbilical Vein Cells Experimental Cell Research. ,vol. 280, pp. 159- 168 ,(2002) , 10.1006/EXCR.2002.5636
Rakesh K. Jain, Emmanuelle di Tomaso, Dan G. Duda, Jay S. Loeffler, A. Gregory Sorensen, Tracy T. Batchelor, Angiogenesis in brain tumours Nature Reviews Neuroscience. ,vol. 8, pp. 610- 622 ,(2007) , 10.1038/NRN2175
Michael Pierce, Chunwei Wang, Mark Stump, Alexander Kamb, Overexpression of the β-catenin binding domain of cadherin selectively kills colorectal cancer cells International Journal of Cancer. ,vol. 107, pp. 229- 237 ,(2003) , 10.1002/IJC.11372
Shigetomo Fukuhra, Atsuko Sakurai, Akiko Yamagishi, Keisuke Sako, Naoki Mochizuki, Vascular endothelial cadherin-mediated cell-cell adhesion regulated by a small GTPase, Rap1. Journal of Biochemistry and Molecular Biology. ,vol. 39, pp. 132- 139 ,(2006) , 10.5483/BMBREP.2006.39.2.132
A. D. Norden, G. S. Young, K. Setayesh, A. Muzikansky, R. Klufas, G. L. Ross, A. S. Ciampa, L. G. Ebbeling, B. Levy, J. Drappatz, S. Kesari, P. Y. Wen, Bevacizumab for recurrent malignant gliomas: efficacy, toxicity, and patterns of recurrence. Neurology. ,vol. 72, pp. 779- 787 ,(2008) , 10.1212/01.WNL.0000304121.57857.38
Carolyn A. Staton, Nicola J. Brown, Gary R. Rodgers, Kevin P. Corke, Simon Tazzyman, James C. E. Underwood, Claire E. Lewis, Alphastatin, a 24-amino acid fragment of human fibrinogen, is a potent new inhibitor of activated endothelial cells in vitro and in vivo. Blood. ,vol. 103, pp. 601- 606 ,(2004) , 10.1182/BLOOD-2003-07-2192
L. Naldini, U. Blomer, P. Gallay, D. Ory, R. Mulligan, F. H. Gage, I. M. Verma, D. Trono, In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral Vector Science. ,vol. 272, pp. 263- 267 ,(1996) , 10.1126/SCIENCE.272.5259.263
Shi-Wen Guo, Hong-Min Che, Wen-Zhi Li, Anti-tumor effect of lentivirus-mediated gene transfer of alphastatin on human glioma Cancer Science. ,vol. 102, pp. 1038- 1044 ,(2011) , 10.1111/J.1349-7006.2011.01879.X
Yueping Li, Shudong Qiu, Liping Song, Qingfeng Yan, Guangxiao Yang, Secretory Expression of p53(N15)-Ant following Lentivirus-Mediated Gene Transfer Induces Cell Death in Human Cancer Cells Cancer Investigation. ,vol. 26, pp. 28- 34 ,(2008) , 10.1080/07357900701681384