Multiparametric In Vitro Evaluation of Cytocompatibility of 1% Strontium-Containing Nanostructured Hydroxyapatite

摘要: Hydroxiapatite (HA), one of the most widely employed bioceramic bone substitutes, when applied on its nanostructured form (nHA) may contribute to achieve a crystalline structure which is closer size and morphology biological apatite. Furthermore, HA might also be doped with several different cations effects including Sr2+. Therefore, biomaterial based containing 1% Strontium (nSrHA) could present interesting properties, as strontium described modulator both osteoblast osteoclast activities, presenting an important regulatory role resorption. However, such modifications affect biocompatibility this material, should accessed initially by in vitro methods. work aimed evaluate nSrHA discs human primary osteoblasts through multiparametric assay assesses simultaneously metabolic activity (XTT assay), membrane integrity (NR test) cell density (CVDE). Extracts nSrHA, latex fragments (positive control), polystyrene beads (negative control) nHA (for comparison) were prepared exposed 104 cells for 24h at 37°C/5% CO2 test plates, according ISO 10993-5:2009, quintuplicates. Cells unconditioned media used experimental control. After exposure, tested viability commercial kit (In Cytotox, Xenometrix, Germany). The positive negative controls presented expected results, validating assay. Both SrnHA considered biocompatible, since after exposure statistically similar In conclusion, synthesized are cytocompatible and, consequently, adequate further tests adhesion, proliferation differentiation.