Involvement of Dynein and Spectrin with Early Melanosome Transport and Melanosomal Protein Trafficking

摘要: Melanosomes are unique membrane-bound organelles specialized for the synthesis and distribution of melanin. Mechanisms involved in trafficking proteins to melanosomes transport mature pigmented dendrites melanocytic cells being characterized, but details about those processes during early stages melanosome maturation not well understood. Early must remain perinuclear area until critical components assembled. In this study, we characterized processing two distinct melanosomal proteins, tyrosinase (TYR) Pmel17, elucidate protein or late steps secretory pathway, respectively, determine mechanisms underlying subcellular localization melanosomes. We used immunological, biochemical, molecular approaches demonstrate that movement depends primarily on microtubules actin filaments. contrast, TYR Pmel17 cytoplasmic dynein its interaction with spectrin/ankyrin system, which is sorting cargo from plasma membrane. These results provide important clues toward understanding events formation transport.